Group D-4

化学構造　(Chemical Structure)

● o-Dichlorobenzene 　（o-ジクロロベンゼン； o-DCB） (1,2-クロロベンゼン）
　　　　CAS：95-50-1 　Industry　 MW: 147.00

AM	Sal.	Max (0.5 mg/plate, ±Ｓ9)	□	1-9)
YM	Yeast	Min (4.0 mM, ±Ｓ9),  2h)	○	10)
MB	Asp	Max (4.0 mM, ±Ｓ9,)	□	11)
DNA	E. coli	Min  (0.2mg/mL,  -S9)	○	3)
DNA	E. coli	Max (0.44 mM)	□	12)
SM	CHO	Max (0.14 mg/mL)	□	13)
CA	CHO	Max (0.14 mg/mL)	□	14)
CA	CHL/IU	Max (0.2mg/ml, ±MＳ9), 6-18h	□	15)
MLA	L5178Y	Min (0.5 μｇ/mL, +S9)	○	16)
MNv	Mice, BM	Min (0.7 g/kg), ip	○	17)
MNv	Mice, BM	Max ( 0.2 g/kg), ip	□	18)
MNv	Rats	Max (1 mg/kg ), sc	□	19)
SLRLv	Drosophila	Max (17000 ppm), ih	□	14)
SLRLv	Drosophila	Max (1500 ppm, fd /50000 ppm, sc )	□	20)
UDSv	Mice	Max (2g/kg ), or	□	21)
SM	Drosophila, eyes	Min (1000 ppm, fd/ 5mM  ih	○	22)

 １）Anderson KJ., et al.: J. Agri. Food Chem.. 20, 649-656 (1972)
  2) Lawlor T. & Haworth SR.: Environ. Mutagen., 1, 143 (1979)
  3) Waters, MD., et al., Basic Life Sciences, 21, 275-320 (1982)                                                              
  4) Simizu, N., et al., Mutat. Res., 116, 217-238.(1983)
  5) Howard, PH:  Fate and exposure data for organic chemicals. Vol I, Lewis Publishers (1989)
  6) Nohmi M., et al., Bult. Natl. Inst.. Hyg. Sci., 103, 60-64 (1985)
  7) Rohm and Hass Co. .Report of the Rohm and Hass Company,  Pensylvania; EPA/OTS Doc. No.878212181 (1979)
  8) Nakamura, S., et ,al., Mut. Res., 192, 239-246. (1987)
  9) Ono, Y. et al. Wat. Sci. Tech., 26, 61-69 (1992)
10) Paolini, M., et al.,  Mutat. Res., 413, 205-217 (1998) 
11) Prasad, I, J. Microbiol, 16, 369-372 .(1970)
12) DeMarini, ,et al.,  Environ. Mol. Mutagen., 19, 98-11 (1992).
13) Bioassay Systems, RPA/OTS Doc. No. 40-8420664, 1-23.(1984)
14) Bioassay Systems, EPA/OTS Do. No 40-8320545, 1-19, 126-148,161-181 (1983)
15) Ishidate MJr.(Ed.): Data Book, Chromosomal Aberration Test In Vitro, LICm Tokyo/ Elsevier, Amsterdam (1988)
16) Myhr, B.C. and Caspracy, W.T, Environ. Mol. Mutagen, 18, 51-83 (1991)                                                    
17) Mohtashamipur, E., et al., Mutagenesis, 2, 111-113 (1987)
18) Shelby, M.D et al., Eviron. Mol. Muta, 21, 160-179. (1993)
19) Rohm and Hass Co. Report of the Rohm and Hass Company, Pensylvania; EPA/OTS Doc. No.878212181 (1979)
20) Foureman, P,  et al.,  Environ. Mol. Mutagen., 23, 51-63 (1994)
21) Miyagawa, M. et al., Mutat. Res., 343, 157-183 .(1995)
22) Vogel EW and Nivard MJM, Mutagenesisi, 8, 57-81 (1993)

  US-NTP Genotoxicity Screening:
 ○ Ames Test: (±S9)：□
 ○ SLRL Test with Drosophila： □
 ○ Mouse Lymphoma Assay (L517Y)：　○
 ○ CA Test with CHO,：　□
 ○ SCE Test with CHO ：　○

 【Note】　（Cited from IARC Monographs Suppl., 6, 1987)

No data were available on the genetic and related effects of this compound in humans. It was not mutagenic to fungi or bacteria. (IARC Monographs, 7, 231; 29, 213)

● p-Dichlorobenzene　（p-ジクロロベンゼン ；p-DCB）(1，4-Dichlorobenzene)
　　　 106-46-7　Industrial   147.00

AM	Sal..	Max (?)	□	1-3)
AM	Sal./E. coli	Min ( 0.1% vapor, ±S9),  spa= 0.1% (Minimam effected dose)	○	10)
AM	Sal..	Max ( 5.0 mg/plate), ±S9	□	12, 13)
AM	Sal..	Min ( 2.5 mg/plate), +S9	○	14)
AM	E. coli	Max (?)	□	4)
MB	A. nidulans	Min (?)	○	15)
SM	CHO	Min ( 0.35mg/ml), +S9, for 4h	○	16)
YM	S. cerevisiae:	Max (?)	□	4)
MB	B. subtilis	Max (?)	□	5)
SLRL	Drosophila	Max (?)	□	5)
CA	CHO	Max (?)	□	6)
CA	CHL/IU	Max （ 0.2 mg/ml, ±MS9),  6-18h	□	7)
CA	CHL/IU	Max （ 0.14 mg/ml, ±S9),  6-18h	□	11)
CA	Human lym./CHO	Max （ 0.5 mg/ml, ±S9), for 4h	□	17)
CA	Rat hepatocytes	Min ( 0.2 mg/ml), -S9), for 48h	□	18)
UM	S. cerevisiae:	Max （ 0.44 mg/ml, ±S9), for 4h	□	19)
SCE	CHO	Max （ 0.15mg/ml, ±S9)	□	20)
SCE	Human lym	Min (  0.05 mg/ml), -S9), for 46h	○	17)
UDS	HeLa	Max （ 0.5mg/ml, ±S9)	□	20)
UDS	Human lym	Max （ 1 mM)	□	21)
CT	Balb/c3T3	Max （ 1.40 mg/ml, ) ,-S9	□	22)
MNs	Mice, BM	Min ( 1.4 g/kg x 2), ip	○w	8)
DLv	Mice,	Max ( 450 ppm, 6h/d, for 5 days), ih	□	28)
MNv	Plant	Min (?)	○	9)
SLRLv	Drosophila	Max ( 15600 ppm),	□	23)
CAv	Rats, BM	Max ( 500 ppm, 5h/d, 5d/w, for 3 mon.), ih	□	24)
MNv	Mice, BM	Max ( 2.5 g/kg) or	□	25)
MNv	Mice, BM	Max ( 2 g/kg x 2), or	□	26)
MNv	Mice Peri .lym	Max ( 1.8. g/kg, for 13w ), or	□	27)
SCGv	Mice , liver	Min ( 2 g/kg), ip	○	29)
UDSv	Mice , liver	Max ( 1. g/kg,), or	□	30)
DNAv	Rats, liver	Min ( 300 mg/kg/d for 2 days), or	○	31)

１）Haworth S., et al.: Environ. Mutagen., 5 (Suppl 1), 3-142 (1983)
2) Shimizu M., et al.: Mutation Res., 116, 217-238 (1983)
3) Simmon VF., et al. : Final Report Contract No. 68-02-2947, SRI Project LSU-7558, US-EPA (1979)
4) Waters MS., et al. : Basic Life Sci., 21, 275-326 (1982)
5) Garrett NE., et al.: Mutation Res., 168, 3081-325 (1986)
6) Sofuni T., et al.: Bull. Natl. Inst. Hyg. Sci. (Tokyo),103, 64-75 (1985) (Tables in English)
7) Ishidate MJr.(Ed.): Data Book, Chromosomal Aberration Testin ｉｎ ｖｉｔｒｏ, ＬＩＣ,Ｔｏｋｙｏ/Ｅｌｓｅｖｉｅｒ, Ａｍｓｔｅｒｄａｍ（1988）
8) Mohtashamipur E., et al.: Mutagenesisi, 2, 111-113 (1987)
9) Ma T., et al.: Mutation Res., 138, 157-167 (1977)
10) Ministry of Labour, Japan: Mutagencitiy Test Data of Exist. Chem. Subst., JETOC (Ed.), Suppl. 3, pp. 172 (2005) (Tables in English)
11) Ministry of Labour, Japan: Mutagencitiy Test Data of Exist. Chem. Subst., JETOC (Ed.), Suppl. 3, pp. 245 (2005) (Tables in English)
12) Winker N.,et al., Arbeitsmed. Sozialmed. Umweltmed., 28, 288-292 (1993)
13) Jones E & FennerLA, Huntington Res. Centre, Ltd., Rep. No. RNP 273/8770 (1987)
14) Anderson D, ICI Rep. No. CTLP/298 (1976)
15) Prasad  I, Can. J. Microbiol., 16, 369-372(1970)
16) Litton Bionetics (1987)
17) Galloway SM, et al., Environ. Mol. Mutagen., 10 (Suppl 10) (1987)
18) Canonero R., et al., Mutaagenesis, 12, 35-39 (1997)
19) Ono Y., et al. Wat. Sci., Technol., 23, 329-358 (1991/1992)
20) RBM  Exp. No. M1030, ed. in Ivrea (1986)
21) Perocco., P., et al., toxicol Lett., 16, 69-75 (1983)
22) Litton Bionetics, Genetics Assay No. E-9419 (1986)
23) Bioassay System Corp., World Rev. Post.  Control, 9, 119-127 .(1982)
24) Anderson D & RichardsonCR, (Unpublished) ICI  Rep., No. CTL/P/293(1976)
25) Herbold A  (Unpublished) Rep. No. 14694, Bayer AG (1986)
26) Morita. T.,  et al., Mutation Res., 389, 3-122 (1997)
27) US-NTP, Rep., NTP TR-319, NIH Pub., No. 87-2575 (1987)
28) Anderson D  & Hodge MCE, (Unpublished) ICI Rep. No. CTL/P/296 (1976)
29) Sasaki YF., et al., Mutation Res., 39, 201-214 (1997)
30) Sherman TH.,et al., Teratog. Carcinog. Mutagen., 18, 309-318 (1998)
31) James NH., et al., Arch Toxicol., 72, 784-990 (1998)

  US-NTP Genotoxicity Screening：
  ○　Ames Test: (±S9)：　□
  ○　MLA (L517Y)：　△
  ○　CA Test with CHO Cells：　□
  ○　SCE Test with CHO Cells：　□
　　　
  ARC Criteria for Carcinogenicity:
  Group 2B (possibly carcinogenic to humans)

  【Note】　（Cited from IARC Monographs Suppl., 6, 1987)

No data were available on the genetic and related effects of this compound in humans. It was mutagenic to fungi but not to bacteria. (IARC Monographs, 7, 231; 29, 215)

AM	Sal.	Min (?), ±S9)	○	3, 4)
AM	E. coli	Max (?),  -S9)	□	5)
UDS	HeLa	Min ( 10-7 M,  +S9)	○	1, 4)
CT	Rat embryo	Min ( 5 μg/ml,　+S9)	○	2)
MNv	Mice, BM	Min ( 1.0 g/kg), po	○	3)
MNv	Mice, Embryo	Min ( 1.0 g/kg), po	○	3)
SCEv	Mice, BM	Min ( 10 mg/kg), ip	○	3)
UDSv	Rat liver	Min ( 500 mg/kg)	○	3)

1) Martin CN., et al., Cancer Res., 38, 2621-2627 (1978)
2) Freeman AE., et al., J. Natl. Cancer Inst., 51, 799 (1973)
3) BUA Report, 30  (1989)
4) IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans 4 (1982)
5) Gangolli S., The Dictinary of Sustances and their Effects, 2nd. Ed, The Rorayl Society of Chemistry (1999)

【Note】　(Cited from CICADs Documents, 2, 1998)

There is convincing evidence that 3,3'-dichlorobenzidine is genotoxic both in vivo and in vitro. In in vitro assays, 3,3'-dichlorobenzidine was mutagenic in Salmonela strains (Garner, et al., 1975), increased the frequency of sister chromatid exchange (Shiraishi, 1986), and unscheduled DNA synthesis in human cells (Martin et al., 1978), and "morphologically transformed" rat embryo cells ( Freeman et al., 1973 ).     In in vivo studies,  3,3'-dichlorobenzidine increased the frequency of micronuclei in the bone marrow cells of mice ( Cihak & Vontorkova, 1987), increased unscheduled DNA synthesis in the liver of rats ( Ashby & Mohammed, 1988), and increased the frequency of chromosomal aberrations in the bone marrow cells of mice (You et al., 1993). References ・Ashby J, Mohammed R (1988) UDS activity in the rat liver of the human carcinogens benzidine and 4-aminobiphenyl, and the rodent carcinogens 3,3'-dichlorobenzidine and Direct Black 38. Mutagenesis, 3: 69-71. ・Cihak R, Vontorkova M (1987) Benzidine and 3,3'-dichlorobenzidine (DCB) induce micronuclei in the bone marrow and the fetal liver of mice after gavage. Mutagenesis, 2: 267-269. ・Freeman A, Weisburger E, Weisburger J, Wolford R, Maryak J, Huebner R (1973) Transformation of cell cultures as an indication of the carcinogenic potential of chemicals. Journal of the National Cancer Institute, 51: 799-808. ・Garner R, Walpole A, Rose F (1975) Testing of some benzidine analogues for microsomal activation to bacterial mutagens. Cancer letters, 1: 39-42. ・Martin C, McDermid A, Garner R (1978) Testing of known carcinogens and noncarcinogens for their ability to induce unscheduled DNA synthesis. Cancer research, 38: 2621-2627. ・Shiraishi Y (1986) Hypersensitive character of Bloom syndrome B-lymphoblastoid cell lines usable for sensitive carcinogen detection. Mutation research, 175: 179-187. ・You Z, Brezzell M, Das S, Espadas-Torre M, Hooberman B, Sinsheimer J (1993) Ortho-substituent effects on the in vitro and in vivo genotoxicity of benzidine derivatives. Mutation research, 319 :19-30.

● 2,4-Dichlorobenzoyl chloride （2，4-ジクロロベンゾイルクロライド）
　     89-75-8　Industry 　 209.46

AM

Sal..

Max ( 1.25 mg/plate, ±S9)

□

1)

1) Ministry of Labour, Japan: Mutagencitiy Test Data of Exist. Chem. Subst., JETOC (Ed.), Suppl. 3, pp. 135 (2005) (Tables in English)
● 2-(4,α-Dichlorobenzyl) pyridine 　　（2-(4，α-ジクロロベンジル）　ピリジン）
         142404-69-1　Industry 　 238.12

CA

CHL/IU

Min ( 0.26 mg/ml,　-S9),  6-18h:

○

1

1) Ministry of Labour, Japan: Mutagenicity Test Data of Exist. Chem. Subst., Suppl. 2, pp..240 (2000) (Tables in English)

● 3,4-Dichloro-1-butene （3，4-ジクロロブテン）
　　   　760-23-6　Industry 　 124.99

AM	Sal.	Min (  0.25 mg/plate, -S9)	○w	1)
CA	CHL/IU	Min ( 0.01 mg/ml,　+S9),  6-18h:	○	1)

1) Ministry of Health, Labour & Welfare, Japan (Ed):Toxicity Testing Reports of Environ. Chemicals, Vol. 4 (1996) (Tables in English)

● Dichlorodifluoromethane　（o-ジクロロジフルオロメタン；CFC-12）
　　　  75-71-8 　Industry/Blowing agent 120.91

AM	Sal.	Max (?), ±S9, (gas exposure)	□	1)
SM	CHO	Max (?), (gas exposure)	□	1)
CT	BHK21	Max (?), (gas exposure)	□	1, 2)
DLv	Rats	Max ( １50 mg/kg/day) po, for several weeks	□	1, 3))

１） IPCS: Environ. Health Criteria, 113, WHO (1990)
2)  Longstaff E; Ann. New York Academy of Sciences, 534, 283-298 (1988)
3)  The Canadian Center for Occup. Health and Safety CCOHS CHEMINFO (1996)

● 1,3- Dichloro-5,5-dimethyl hydantoin （1，3-ジクロ-5，5-ジメチルヒダントイン；　DCDMH　）
　　　 118-52-5　　Industry 197.02

US-NTP Genotoxicity Screening：
○ AmesTest ：　□
○ Reciprocal Translocation Test in Drosophila： □
○ MLA Test in Mice：　○
○ CA Test with CHO Cells：　□
○ SLRL Test with Drosophila：　△

● 1,1-Dichloroethane　（1，1-ジクロロエタン） (Ethylidene chloride)
　　　  75-34-3　　Industrial 　 98.96

AM	Sal.	Max ( 5.0mg/plate, ±S9)	□	1,2,3)
AM	Sal.	Min (?)	○	4)
MB	A. nidulans　 （コウジカビ）	Min (?) (Non-disjunction)	○	5)
CA	CHO	Max (?)	□	6)
CA	CHL/IU	Max ( 1.0mg/ml/-MS9； 11.7mg/ml, +MS9)	□	7)
SCE	CHO	Min (?)	○	6)
DNA	Rats & Mice/ hepatocytes	Min (?)	○	4,8)
CT	BALB/c 3T3	Max ( 1.0mg/ml/-MS9； 11.7mg/ml, +MS9)	□	9,10)
Others	SH/embryo	Min ( 1.0mg/ml/-MS9； 11.7mg/ml, +MS9)	○	11)
Others	V79	Min (?) (Metabolic cooperation)	○	12)

1) Simmon VF., et al.: Dev. Toxicol. Environ. Sci., 2, 249-258 (1977)
2) Nohmi T., et al.: Bull. Natl. Inst. Hyg. Sci. (Tokyo), 103, 60-64 (1985)
3) Ishidate MJr (Ed.) Data Book of Mutagenicity Tests on Chemicals in Bacteria, LIC/Japan (1991) (Tables in English)
4) Milman HA., et al.: Ann. N.Y. acad. Sci., 534, 521-530 (1988)
5) Crebellei R., et al.: Mutatation Res., 201(2), 401-411 (1988)
6) NTP: Fiscal Year 1988, Public Health Service, USA (1988)
7) Ishidate MJr.(Ed.): Data Book, Chromosomal Aberration Test In Vitro LIC, Tokyo/Elcevier, Amsterdam (1988)
8) Williams GM., et al.: Mutation Res. 221, 263-286 (1989)
9) Tu AS., et al.: Cancer Lett. 28, 85-92 (1985)
10) Hach GG., et al.: Cancer Res., 43,1945-1959 (1983)
11) Elmore E., et al.: Abstract No. 655, Toxicologist 7, 164 (1987)
12) Colacci A., et al.: Pharmacol. 49(2),243-254 (1985)
　　　 　
US-NTP Genotoxicity Screening:：
 ○ Ames Test： 　□
 ○ CA Test with CHO Cells：　□
 ○ SCE Test with CHO Cells　○

● 1,2- Dichloroethane　（1，2-ジクロロエタン）　　
　　　  107-06-2　Industry 　 98.96

AM	Sal.	Max (  5.0 mg/plate. ±S9)	□	1)
AM	Sal./E. coli	Min (  0.313 mg/plate, +S9)	○	4)
CA	CHL/IU	Min (  1.0 mg/ml, +S9), 6-18h; 　D20= 0.52; TR= 28	○	2)
MNv	Mice (ICR)	Max(  360 mg/kg, ip)	□	3)

1) Ishidate MJr (Ed): Data Book for Mutagencity Tests on Chemicals in Bacteria, LIC/Tokyo (1991) (Tables in English)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998) (Tables in English)
3) Morita T et al., Mutation Res., 389, 3-122 (1997)
4) Ministry of Labour, Japan: Mutagencitiy Test Data of Exist. Chem. Subst., JETOC (Ed.), Suppl. 3, pp. 174 (2005) (Tables in English)

【Note】　(Cited from CICADs Documents,1, 1998))

1,2-Dichloroethane has been consistently demonstrated to be genotoxic in numerous in vitro and in vivo assays for a wide range of end-points. It has been mutagenic in Salmonella typhimurium, especially in the presence of an exogenous activation system (Milman et al., 1988; Barber et al., 1981; Moriya et al., 1983 ) , and induces unscheduled DNA synthesis (Williams et al., 1989; Milman et al., 1988), induces gene mutation (,Tan & Hsie, 1981) and forms adducts with DNA in mammalian cells in vitro (Banerjee, 1988). It binds to DNA in all reported in vivo studies in rats and mice (Prodi et al., 1986).  1,2-Dichloroethane has also induced somatic cell and sex-linked recessive lethal mutations in Drosophila (Nylander et al., 1978; Kramers et al., 1991). Available data on genotoxicity are consistent with the hypothesis that the glutathione pathway of conjugation (i.e. production of the glutathione episulfonium ion) is probably of greater significance than the P-450 pathway as the major route for DNA damage (Guengerich et al., 1980; Rannug, 1980; Sundheimer et al., 1982; Inskeep et al., 1986; Koga et al., 1986; Simula et al., 1993); mutation frequency in human cell lines has been correlated with variations in levels of glutathione- S-transferase activities (Crespi et al., 1985). References ・Banerjee S (1988) DNA damage in rodent liver by 1,2-dichloroethane, a hepatocarcinogen. Cancer biochemistry and biophysics, 10:165-173. ・Barber RD, Donish WH, Mueller KR (1981) A procedure for the quantitative measurement of the mutagenicity of volatile liquids in the Ames Salmonella microsome assay. Mutation research, 90:31-48. ・Crespi CL, Seixas GM, Turner TR, Ryan CG, Penman BW (1985) Mutagenicity of 1,2-dichloroethane and 1,2-dibromoethane in two human lymphoblastoid cell lines. Mutation research, 142:133-140. ・Guengerich FP, Crawford WM, Domoradzki JY, MacDonald TL, Watanabe PG (1980) In vitro activation of 1,2-dichloroethane by microsomal and cytosolic enzyme. Toxicology and applied pharmacology, 55:303-317. ・Inskeep PB, Koga N, Cmarik JL, Guengerich FP (1986) Covalent binding of 1,2-dihaloalkanes to DNA and stability of the major DNA adduct, S-[2[(N7-guanyl)ethyl]glutathione. Cancer research, 46:2839-2844. ・Koga N, Inskeep PB, Harris TM, Guengerich FP (1986) S-[2-(N7-guanyl)ethyl] glutathione, the major DNA adduct formed from 1,2-dibromoethane. Biochemistry, 25:2192-2198. K ・Kramers PG, Mout HC, Bissumbhar B, Mulder CR (1991) Inhalation exposure in Drosophila mutagenesis assays: experiments with aliphatic halogenated hydrocarbons, with emphasis on the genetic activity profile of 1,2-dichloroethane. Mutation research, 252:17-33. ・Milman HA, Storey DL, Riccio ES, Sivak A, Tu AS, Williams GM, Tong C, Tyson CA (1988) Rat liver foci and in vitro assays to detect initiating and promoting effects of chlorinated ethanes and ethylenes. Annals of the New York Academy of Sciences, 534:521-530. ・Moriya M, Ohta T, Watanabe K, Miyazawa T, Kato K, Shirasu Y (1983) Further mutagenicity studies on pesticides in bacterial reversion assay systems. Mutation research, 116:185-216. ・Nylander P-O, Olofsson H, Rasmuson B, Svahlin H (1978) Mutagenic effects of petrol in Drosophila melanogaster. I. Effects of benzene and 1,2-dichloroethane. Mutation research, 57:163-167. ・Prodi G, Arfelini G, Colacci A, Grilli S, Mazzulo M (1986) Interaction of halocompounds with nucleic acids. Toxicologic pathology, 14:438-444. ・Rannug U (1980) Genotoxic effects of 1,2-dibromoethane and 1,2-dichloroethane. Mutation research, 76:269-295. ・Simula TP, Glancey MG, Wolf CR (1993) Human glutathione S-transferase-expressing Salmonella typhimurium tester strains to study the activation/detoxification of mutagenic compounds: studies with halogenated compounds, aromatic amines and aflatoxin B1. Carcinogenesis, 14:1371-1376. ・Sundheimer DW, White RD, Brendel K, Sipes IC (1982) The bioactivation of 1,2-dibromoethane in rat hepatocytes: covalent binding to nucleic acids. Carcinogenesis, 3:1129-1133. ・Tan E-L, Hsie AW (1981) Mutagenicity and cytotoxicity of haloethanes as studied in the CHO/HGPRT system. Mutation research, 90:183-191. ・Williams GM, Mori H, McQueen CA (1989) Structure-activity relationships in the rat hepatocyte DNA-repair test for 300 chemicals. Mutation research, 221:263-286.

● 1,2-Dichloro-1-ethoxyethane （1,2-ジクロロ-1-エトキシエタン）
        623-46-1　Industry 　　143.0

CA

CHL/IU

Min (  0.0015 mg/ml, -S9), 24h; D20=0.0012;

◎

１)

1) Ministry of Labour, Japan: Mutagenicity Test Data of Exist. Chem. Subst.,, pp..430 (1996) (Tables in English)

● 1,2- Dichloroethylene （1，2-ジクロロエチレン；1，2- DCE）　　
　　 　(cis); 　　156-59-2  (sym); 　 540-59-0; 　(trans)　  156-60-5 　　　Industry 96.94

AM	Sal.	Max ( 0.05 ml/plate, -S9)	□	1,)
AM	Sal.	Max ( 10 mg/plate, ±S9 )	□	2,)
AM	E. coli	Max ( 0.28 mg/ml, ±S9 )	□	3)
YM	S. cerevisiae	Min ( 0.97 mg/ml, +S9 )	○w	4)
YM	S. cerevisiae	Max ( 0.97 mg/ml, ±S9 )	□	5, 6)
CA	CHL/IU	Max ( 2.0 mg/ml, ±S9 )	□	7)
CA	CHO	Mix ( 5.0 mg/ml, -S9 )	○	2, 8)
HMA	S. cerevisiae/mice	Min ( 1.3 g/kg, or )	○	4)
HMA	Sal./ mice	Min ( 0.5 x LD50, or )	○	1)
CAv	Mice, BM	Min ( 0.5 x LD50, ip )	△	1)

1) Cerna M & Kypenova H., Mutation Res., 46, 214-215 (1977)
2) USA-NTP, Toxicity Rep. Ser., No. 55 (2001)
3) Greim H., et al., Biochem. Pharmacol., 24, 2013-2017 (1975)
4) Bronzetti G., et al., Teratog. Carcinog. Mutag., 4, 365-375 (1984)
5) Cantelli-Forti G & Bronzetti G, Ann. N,Y. Acad. Sci., 534, 679-693 (1988)
6) Galli A., et al., Bull. Sot. Ital., Biol.. sper., 58, 860-863 (1982)
7) Sawada M., et. al., Mutation Res., 187, 157-164 (1987)
8) Galloway SM., et al., Environ. Mol. Mutagen., 10, 1-175 (1987)

  US-NTP Genotoxicity Screening：
  ○ Ames Test :　□
  ○ CA Test with CHO : （cis-) & (trans-) □　　
  ○ SCE Test with CHO : (cis-)○ (trans-) □

● cis-1,2-Dichloroethylene 　　（cis-1,2-ジクロロエチレン）
　　 　156-59-2 　Industry 　 96.94

AM	Sal.	Max (  5.0 mg/plate, ±S9)	□	1)
CA	CHL/IU	Max ( 7.5 mg/ml,　±MS9),  6-18h:	□	2)

1) Ishidate MJr (Ed.) Data Book of Mutagenicity Tests on Chemicals in Bacteria, LIC/Japan (1991) (Tables in English)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998) (Tables in English)

● Dichloroethyl ether （ジクロロエチルエーテル）
　　　　111-44-4　(Industrial /Solvent) 143.01
　　　　
  US-NTP Genotoxicity Screening：
  ○ AmesTest :　○w
  ○ SLRL Test with Drosophila:　○
  ○ Reciprocal Translocation Test with Drosophila: □

 IARC Criteria for Carcinogenicity：
  Group 3 （Not classifiable as to its carcinogenicity to humans）

●Top Page　（トップページ）

●Abbreviation 　（省略記号）

●Mutagenicity （変異原性）

●Test Systems　(試験法の種類）

●Technical Problems　（技術的問題点）

●List of　Compounds（化合物リスト）

●Evaluation of Results　（試験結果の評価）