| Diglyme was not mutagenic in several Ames tests with or without S9 mix (Hoechst, 1979; McGregor et al., 1983; Mortelmans et al., 1986).
Diglyme also had no effect in a test for unscheduled DNA synthesis
in human embryo fibroblasts (McGregor et al., 1983).
Diglyme did not induce chromosomal aberrations in bone marrow cells
in groups of 10 male and 10 female CD rats exposed to 250 or 1000 ppm (1395
or 5580 mg/m3) diglyme 7 h/day for 1 or 5 days (McGregor et al., 1983). A dominant lethal test is described in section 220.127.116.11 (McGregor
et al., 1983). The reduced number of pregnancies and an increase in preimplantation
losses may be due either to a dominant lethal effect or to reduced fertility
of the males. Considering the known effects of diglyme on fertility, the
authors of the study assume that reduced fertility is a cause of the effects.
Also, the post-implantation losses may be due to reduced fertility instead
of a dominant lethal effect, as it is known that early deaths may be a
consequence of a low number of implantations.
A recessive lethal test on Drosophila melanogaster exposed to 250 ppm
(1395 mg/m3) for 2.75 h could not be evaluated because of an unusually high death rate in a control group (McGregor et al., 1983).
E McGregor DB, Willins MJ, McDonald D, Holmstrom M, McDonald D, Niemeier
RW : Genetic effects of 2-methoxyethanol and
bis(2-methoxyethyl)ether. Toxicology and applied pharmacology, 70: 303-316. (1983)
E Mortelmans K, Haworth S, Lawlor T, Speck W, Tainer B, Zeiger E
(1986) Salmonella mutagenicity tests: II. Results from the testing of 270 chemicals.
Environmental mutagenesis, 8(S7): 1-119 (1986)