● 1,2-Dicyanobenzene （1，2-ジシアノベンゼン）(BCN)
　　　　91-15-6　 Industry　 128.13

AM	Sal./E.coli	Max （ 5.0 mg/plate, ±S9）	□	1)
CA	CHL/IU	Min( 0.65 mg/ml, +S9), 6-18h; (Poly)	○	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 8 (2001) (Tables in English)

● 1,3-Dicyanobenzene（1，3-ジシアノベンゼン）
　　　　 626-17-5　 Industry　 128.13

AM	Sal/E.coli	Max（ 5.0 mg/plate, ±S9）	□	1)
CA	CHL/IU	Max( 1.3 mg/ml, ±S9), 6-18h	□	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 4 (1996) (Tables in English)

● 1,4-Dicyanobenzene（1，4-ジシアノベンゼン）
　　　 623-26-7　 Industry　 128.14

AM	Sal./E.coli	Max（ 5.0 mg/plate, ±S9）	□	1)
CA	CHL/IU	Max( 1.3 mg/ml, ±S9), 6-18h	□	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 3 (1996) (Tables in English)

● Dicyclohexylamine（ジサイクロヘキシラミン）
　　　 101-83-7　 Industry　 181.32

AM	Sal./E.coli	Max（ 5.0 mg/plate, ±S9）	□	1)
CA	CHL/IU	Max( 2.2 mg/ml, +S9), 6-18h	△	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 6 (1998) (Tables in English)

【Note】 (Cited from IARC Monograph Suppl., 6 (1987)

No data were available on the genetic and related efects of this agent in humans. 　　It induced CAs in cultured human lymphocytes, It was not mutagenic to bacteria.

● N, N-Dicyclohexyl-2-benzothiazolesulfenamide（N, N-サイクロヘキシル-
　　　2-ベンゾチアゾールスルフェナミド）
　　　 4979-32-2 　Industry　 346.59

AM	Sal./E.coli	Max（ 5.0 mg/plate, ±S9）	□	1)
CA	CHL/IU	Max( 0.82 mg/ml, -S9), 24h; (also Poly)	△	1)
MN	CHL/IU	Min( 0.21mg/ml, -S9), 48h	○	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 3 (1996) (Tables in English)

● Dicyclohexylcarbodiimide （ジサイクロヘキシルカルボジイミド）
　　　 538-75-0 　Industry　 206.33

AM	Sal./E.coli	Max（ 2,5 mg/plate, ±S9）	□	1)
CA	CHL/IU	Max( 0.03 mg/ml, ±S9), 6-18h	□	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol.1 (1994) (Tables in English)

● Dicyclopentadiene （ジサイクロペンタジン）
　 　　 77-73-6　　Industrial 　 132.22

AM	Sal./E.coli	Max（ 5.0 mg/plate, ±S9）	□	1)
CA	CHL/IU	Max( 0.10mg/ml,　6-18h, +S9)	△	1, 2)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 3 (1996) (Tables in English)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro, LIC, Tokyo (1998) (Tables in English)

US-NTP Genotoxicity Screening：
○AmesTest：　□

● Dicyclopentadienytl iron　（ジシクロペンタジェニル鉄；フェロセン）
　　　　 102-54-5　Industrial /Catalyst 175.96

SLRL

Drosophila

Min ( 30% of lethal dose), 24h

○

1)

１）Zimmering S., et al.: Environ. Mutagenesis, 7, 87-100 (1985)
　　
US-NTP Genotoxicity Screening：
○ AmesTest：　△
○ CA Test with CHO Cells ：　□
○ SCE Test with CHO Cells:：　○
○ Reciprocal Translocation Test with Drosophila：　○

● Dicyclopentylsilanediol （ジシクロペンチルシランジオール）
　　　 211495-85-1　　Industry　 200.35

AM	Sal./E.coli	Max（2,5 mg/plate, ±S9）	□	1)
CA	CHL/IU	Max( 0.3 mg/ml, ±S9), 6-18h	□	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 8(2) (2001) (Tables in English)

● Dienochlor　（ジノクロール）
　　　 2227-17-0　Pesticide 　 474.6

REC	B. subtilis	Max ( 0.01 mg/disk)	□	1)
AM	Sal/E. coli	Max ( 5,0 mg/plate, ±S9)	△	1)

1) Agro Kane Sho, Co. Ltd.: J. Pesticide Sci., 15, 511-514 (1990) 　

● Dieldrin　（ディルドリン）
　　　 60-57-1　　Insecticide　　　 380.93

AM	Sal.	Max (?)	□	1)
CA	WI-38	Min( 1μg/ml,−S9), 24h	○	2)

１） McCann J., et al. : Proc. Natl Acad. Sci. (USA), 72, 5135-5939 (1975)
 2) Majumdar SK., et al.: J. Hered., 67, 303-307 (1976)
　　　
US-NTP Genotoxicity Screening：
○ AmesTest：　□
○ Mouse Lymphoma Assay (L517Y)：　○
○ CA Test with CHO Cells：　□
○ SCE Test with CHO Cells：　○

【Note】(Cited from IARC Monograph Suppl., 6 (1987)

In one study, CAs were not found in peripheral blood lymphocytes of workers exposed to this agent. 　　It did not induce DL mutation in mice or CAs in bone-marrow cells of Chinese hamsters treated in vivo. It induced UDS in transformed human fibroblasts but not in rat hepatocytes; it did not induce single-strand breaks in Chinese hamster V79 cells. It inhibited intercellular communication in human and rodent cell systems. it did not induce SLRL mutation in Drosophila, was not mutagenic to bacteria and did not induce breakage of plasmid DNA.

● 1,2: 3,4-Diepoxybutane (DBE) （1,2: 3,4-ジエポキシブタン）
　　　 1464-53-5　　Industry/Laboratory　　　 86.09

DNA	E. coli (repair)	Min ( 50 μg/well)	○	1)
YM	S. serev.	Min ( 5x10 -3M)	○	2)
CA	Vicia faba	Min ( 4x10 -5M)	○	3)
PLNv	Maise	Min ( 0.2%)	○	4)
CT	Syr. Ham.	Min ( 0.05 μg/ml)	○	5)
MNv	Mice (CD-1)	Min ( 36 mg/kg, po)	○	6)

1) Ichinotusubo D., et al., Mutation Res., 46, 53-62 (1977)
2) Zimmermann, FK: Mutation Res., 11, 327-337 (1969)
3) Swietlinska Z: Mutation Res., 16, 41-47 (1972)
4) Krizinger D: Genetics, 45, 143-154 (1960)
5) Pienta RJ et al: Int. J. Cancer, 19, 642-655 (1977)
6) Morita T., et al., Mutation Res., 389, 3-122 (1997)

● Diethanolamine　（ディエタノールアミン, DEA）　
　　　 111-42-2　　Detergent　　　 105.14

AM	Sal.	Max (?)	□	1-3)
MLA	L5178Y	Max (?)	□	4)
YM	Yeast	(Mitotic gene conversion) Max (?)	□	3)
CA	CHO	Max (?)	□	5)
SCE	ＣＨＯ	Max (?)	□	5)
CA	Rat RＬ１	Max (?)	□	3)
CT	Ham/Embryo	Max (?)	□	6)
Other	(NBP-Test)	( Reaction with 4-（p-nitrobenzyl）pyridine), Max (?)	□	1)

１）Hedenstedt A.: Mutation Res., 53, 198-199 (1978)
2) Haworth S., et al.: Environ. Mutagen. 5 (Suppl. 1), 1-142 (1983)
3) Dean BJ., et al.: Mutation Res.., 153, 57-77 (1985)
4) Myhr BC., et al.: Environ. Mutagen. 8 (Suppl. 6), 58-93 (1986)
5) Loveday KS., et al.: environ. Mol. Mutagen., 13, 69-94 (1989)
6) Inoue K., et al.: Mutation Res., 101, 305-313 (1982)

● Diethanolnitrosamine　（ジエタノールニトロサアミン） (See also N-5)
　　　　 1116-54-7　 Laboratory　 134.13

AM	Sal.	Max (?)	□	1)
CA	CHL/IU	Min( 5.0 mg/ml,−S9), 24h; D20= 4.3; TR= 4.0	○	2)

１） (?)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test i In Vitro, LIC, Tokyo (1998) (Tables in English)

● Diethylamine　　（ジエチルアミン；DEN）
　　106-46-7　 Industry　　 37.14

AM	E. coli	Max (?)	□	1,2)
MB	C. intermedia （カンジダ）	Max (?)	□	3)
MB	K.rhinosclerom. （鼻硬腫菌）	Max (?)	□	4)
DLv	Rats	Max (?)（ih)	□	5)
MNv	Rats	Max (?)	□	5)
UDSv	Rat/Kidney	Max (?)	□	6)
CA	Vicia fabia (ソラマメ）	Min(?)	○	7)

１）Hedenstadt A.: Mutation Res., 53, 198-199 (1978)
2) Zeiger E., et al.: Environ. Mutagen., 9 (Suppl 9), 1-110 (1987)
3) Ali AMM., et al.: Egypt. J. Microbiol., 19, 89-94 (1984)
4) Ali AMM,, et al.: Egypt. J. Microbiol., 14, 1-2 (1979)
5) Legator M., et al.: Environ. Res., 20, 99-124 (1979)
6) Loury DJ., et al.: Toxicol. Appl. Pharmacol. 87, 127-140 (1987)
7) Galal HE. & Abd-Alla SA.: Egypt J. Genet. Cytol. 5, 262-280 (1976)　　

US-NTP Genotoxicity Screening：
　○Ames Test：　□

● 2-Diethylaminoethanol 　（2-ジエチルアミノエタノール；　DEAE）
　　　 100-37-8　 　Industry　　　 117.19

AM

Sal

Max (?)

□

1)

１） Zeiger E. et al.: Environ. Mutagen., 9 (Suppl. 9), 1-110 (1987)

US-NTP Genotoxicity Screening：
　○ AmesTest ：　□

● 2-(Diethylamino) ethyl methacrylate（2-(ジエチルアミノ)エチルメタクリラート）
　　　 105-16-8 　Industry　 185.27

AM	Sal./E.coli	Max（ 5.0 mg/plate, ±S9）	□	1)
CA	CHL/IU	Min( 0.3 mg/ml, +S9), 6-18h; (Poly also)	○	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 6 (1998) (Tables in English)

● N,N'-Diethylaniline　(DEA)
　　 91-66-7　Industry　 149.24

AM

Sal/E.coli

Max（ 0.156 mg/plate, ±S9）

□

1)

１）Ministry of labour, Japan: Mutagenicity Test Data of Exist. Chem. Subst., Suppl (1997) (in Japnese) (Tables in English)

● 1,4-Diethylbenzene （1，4-ジエチルベンゼン）
　　 105-05-5　Industry　 134.22

AM	Sal./E. coli	Max ( 0.078 mg/plate, ±S9)	□	1)
CA	CHL/IU	Max ( 1.13 mg/mg, ±S9), 6-18h	□	1)

１）Ministry of Health & Welfare, Japan (Ed): Toxicity Testing Reports of Environ. Chemicals, Vol. 2 (1995) (Tables in English)

● Diethylene glycol （ジエチレングリコール）
　　　 111-46-6 　 Industry　　　 106.12

AM	Sal.	Max　(?)	□	1)
CA	CHL/IU	Max ( 40 mg/mg), ±S9 （Positive at only at very high dose)	△	2)

１）Zeiger E. et al.: Environ. Mutagen., 9 (Suppl. 9), 1-110 (1987)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro, LIC, Tokyo (1998) (Tables in English)

● Diethylene glycol dimethyl ether （ジエチレン　グリコール ジメチルエーテル）(Diglyme)
　　　 111-49-6 　 Industry　　　 134.17

【Note】　(Cited from CICADs Documents,1, 1998))

Diglyme was not mutagenic in several Ames tests with or without S9 mix (Hoechst, 1979; McGregor et al., 1983; Mortelmans et al., 1986).   Diglyme also had no effect in a test for unscheduled DNA synthesis in human embryo fibroblasts (McGregor et al., 1983).   Diglyme did not induce chromosomal aberrations in bone marrow cells in groups of 10 male and 10 female CD rats exposed to 250 or 1000 ppm (1395 or 5580 mg/m3) diglyme 7 h/day for 1 or 5 days (McGregor et al., 1983).   A dominant lethal test is described in section 8.7.1.1 (McGregor et al., 1983). The reduced number of pregnancies and an increase in preimplantation losses may be due either to a dominant lethal effect or to reduced fertility of the males. Considering the known effects of diglyme on fertility, the authors of the study assume that reduced fertility is a cause of the effects. Also, the post-implantation losses may be due to reduced fertility instead of a dominant lethal effect, as it is known that early deaths may be a consequence of a low number of implantations.   A recessive lethal test on Drosophila melanogaster exposed to 250 ppm (1395 mg/m3) for 2.75 h could not be evaluated because of an unusually high death rate in a control group (McGregor et al., 1983). References ・ McGregor DB, Willins MJ, McDonald D, Holmstrom M, McDonald D, Niemeier RW : Genetic effects of   2-methoxyethanol and      bis(2-methoxyethyl)ether. Toxicology and applied pharmacology, 70: 303-316. (1983) ・ Mortelmans K, Haworth S, Lawlor T, Speck W, Tainer B, Zeiger E (1986) Salmonella mutagenicity tests: II. Results from the testing of 270 chemicals. Environmental mutagenesis, 8(S7): 1-119 (1986) ・　Hoechst (1979)　(unpublished)