A-5

　　　　　　　　　　　　　　　　　　　　　　　　

Group A-5 化学構造　(Chemical Structure)

● Allyl chloride （アリルクロライド; 塩化アリル）(3-Chloro-1-propene)
　　CAS: 107-05-1 Industrial /Intermediate　 MW: 76.5

AM Sal. Min ( 2.35 mg/plate, ±S9) ○ 1-5)

AM Sal./E. coli Min ( 2.5 mg/plate, -S9) ○ 8)

CA CHL/IU Min ( 0.4 mg/ml, -S9):, 24h; D20=0.37, TR=86 ○ 7)

MB E. coli Min (?) ○ 1)

YM Yeast Min (18.4 x 10^-5M, -S9) ○ 6)

MB S.coelicolor Min (4.7 mg/plate, -S9) ○ 4)

MB A.nidulans Max ( 18 mg/plate) □ 4)

UDS HeLa Cells Min ( 10^-3 mol/L) ○ 5)

DNA E.coli Min ( 10 μl) ○ 6)

SLRLv Drosophila Max ( 150 ppm, ih, for 7h □ 9)

DLv Rats Min ( 25 ppm, ih, for 7h/d, for 5d.) ○ 9)

CAv Rats Max ( 25 ppm, ih, for 7h/d, for 5d.) □ 9)

1) Dean BJ, et al., Mutation Res. 153,57 (1985)
2) Neudecker T & Henschler D: Mutation Res., 170, 1 (1986)
3) Eder E, et al.: Biochem. Pharmacol., 29, 993 (1980); IAIC; Monograph, Vol. 36, pp 3954 (1985)
4) Bignami M, et al.: Chemi. Biol Interact. 30, 9 (1980)
5) Schiffmann D, et al.: Cancer Lett., 20, 263 (1983)
6) McCoy E., et al.: Mutation Res., 57, 11-15 (1978)
7) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1997) (Tables in English)
8) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) Suppl. 3., pp. 152 (2005) (Tables in English)
9) McGregor DB, Invesk, Res. Internat., Ltd., Rep. NTISPB 86-239845 (1981)

US-NTP Genotoxicity Screening
○　:Ames Test: ○
○　:CA Test (CHO)○
○　SCE (CHO)Test: ○
○　Drosophila (RT) Test: □

● Allyl glycidyl ether　(AGE)　（アリルグリシディルエーテル）
　 (3-Allyloxy-1,2-epoxypropane)
　 106-92-3　　　Industry　　　114.14

AM Sal. Min ( 10 mg/plate), -S9 ○ 1)

AM Sal. Min ( 10 mg/plate), ±S9 ○ 2,3)

AM Sal./E. coli Min ( 1 mg/ml), ±S9 ○ 4)

AM Pneuumococ. Min ( 0.057 mg/ml), -S9 ○ 5)

CA CHO Min ( 0.15 mg/ml), ±S9 ○ 6)

CA Rat, RL4 cells Min ( 0.037 mg/ml), -S9 ○ 4)

SCE CHO Min ( 0.1 mg/ml), ±S9 ○ 3)

SCE V79 Min ( 0.822 mg/ml), -S9, for 2h ○ 6)

UDS Hum. leuko. Min ( 0.5 mg/ml), -S9, for 4.5h ○ 7)

YM S. cerevisiae Min ( 0.005 mg/ml), ±S9 ○ 4)

CT C3H/10T1/2 Min ( 1000 ppm) -S9, for 21d. ○ 8)

CA CHL/IU Min ( 0.1 mg/ml, -S9), 24h; D20=0.08; TR=140 ○ 9)

SLRLv Drosophila Min ( 5500 ppm, or), for 3d. ○ 3, 10)

CAv Drosophila Min ( 5500 ppm, fed), for 3d. ○ 11)

MNv Mice Max ( 10 ppm, ih, 6h/d, 5d/w), for 102w, ih □ 12)

DLv Mice Max ( 2 g/kg, 3d/w, sc), for 8w □ 7)

1) Wade MJ, et al.: Mutation res., 66, 367-371 (1979)
2) Canter DA, et al.: Mutation Res., 172, 105-138 (1986
3) US-NTP, Tech. Rep. Ser., No. 376 (1990)
4) Shell oil, Rep. No 258, Shell Res. Ltd, US-NTP/OPTS, Fiche #: OTSO513375 (1984)
5) Voogd CE. et al.: Mutation Res., 89, 269-282 (1981)
6) Von Der Hude W., et al., Mutation Res., 249, 55-70 (1991)
7) Dow Chem., EPA/OTS: DOC # 878211534 (1982)
8) Kowalski et al., Envron. Mol. mutagen, 37, 231-140 (2001)
9) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1996) (Tables in English)
10)Yoon JS, et al.: Environ. Mutagen., 7, 349-367 (1985)
11)Zimmering S: Environ. Mutagen., 5, 907-921 (1983)
12)Witt K., et al., Environ. Mol. Mutagen., 36, 163-194 (2000)

● Allylisothocyanate (AITC)
47-06-7 Industry 99.16

AM Sal. Min (?) ○ 1)

DNA E.coli Min (?) ○ 1)

MN Human HepG2 Min (?)　 ○w 1)

HM E.coli/ mice Min ( 90 mg/kg ) ○w 1)

UDSv Rat liver Max ( 125 mg/kg, or) □ 2)

CA CHO Max (?) □ 3)

SCE CHO Max (?) □ 3)

CA Indian munthac Max (?) □ 4)

MLA L5178Y Min (?), 4h ○ 5)

CT Ch. B241 Min ( 5 nM) ○ 6)

AM Sal. Min (?), +S9 ○ 7)

UDS HeLa cells Max (?) □ 8)

MB E.coli, WP67 Min (?), +S9 ○ 9)

1) Kassie F & Knasmuller S., Chemi. Biol. Interact., 127 (2), 163-180 (2000)
2) Bechte D., et al., Terat. Carci. Mutagen, 18 (5), 209-217 (1998)
3) Muck SR, et al., Mutation Res., 348 (1), 19-23 (1995)
4) Muck SR & Johnson IT, Mutation Res., 300 (2) 111-117 (1993)
5) McGregor DB, et al., Environ. Mol. Mutagen., 12 (1), 85-154 (1988)
6) Ksamaki A., et al., J. Toxicol., Sci., 12 (4), 383-396 (1987)
7) Neudecker T & Henschler D, Mutation Res., 156 (1-2), 33-37 (1985)
8) Schiffmann D, et al., Cancer Lett., 20 (3), 263-269 (1983)
9) Rihova E, Folia Microbiol.(Praha), (1) 25-31 (1982)

【Review】 IARC Monogr. Evaluat. Carcinog. Risk. Chem. Hum., 36, 55-68 (1985)

● Allylpropyl disulfide（アリルプロピルジサルファイド）
2179-59-1　Flavor/Food Additive　　148.16

US-NTP Genotoxicity Screening：
○ Ames Test: □

● Aluminium chloride (anhydrous) (無水塩化アルミニウム）
　 7446-70-0 133.34

AM Sal./E.coli Min ( 2.0 mg/plate、±S9）;Spa=60.5 ○ 1)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1996) (Tables in English)

● Aluminium potassium sulfate (Burnt alum)
　 10043-67-1　　Industry/Food　　258.19

CA CHL/IU Max ( 1.0 mg/ml,-S9), 24-48h　　 □ 1)

1) Sofuni T (Ed.): Data Book of Chromosomal Aberration Test in vitro, LIC, Tokyo (1998） (Tables in English)

● Amaranth (アマランス）（C.I.16185; Food Red. No.2）
　 915-67-3　　　Food 604.48

AM Sal. Max (?) □ 1)

CA CHL/IU Min ( 0.25 mg/ml, -S9), 24h D20=0.46: TR=24 ○ 2, 3)

1) Fujita H., et al., Ann. Rep. Tokyo Metr. Res. Lab. P.H., 39, 343-350 (1988) (Tables in English)
2) Ishidate MJr & Odashima S: Mutation Res., 48, 337-354 (1977)
3) Sofuni T.(Ed.): Data Book of Chromosomal Aberration Test in vitro LIC, Tokyo (1998) (Tables in English)

● Ametryne （アメトリン）
　 834-12-8　　Agriculture　　227.33

CA CHL/IU Max ( 0.25 mg/ml, -S9), 24-48h □ 1)

1) Sofuni T.(Ed.): Data Book of Chromosomal Aberration Test in vitro LIC, Tokyo (1998) (Tables in English)

● 9-Aminoacridine- HCl 　(Acramine Yellow)
134-50-9　　Medicine　　230.71　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　

CA CHL/IU Min ( 0.75 μg/ml, -S9), 24-48h; D20=0.0006 mg/ml; TR=65000 ◎ 1)

1) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test in vitro LIC, Tokyo (1998) (Tables in English)

● 1-Aminoanthraquinone （1-アミノアントラキノン）
　 82-45-1　　Industry　　258.19

AM Sal./E.coli Min ( 312.5 μg/plate、+S９） ○ 1)

CA CHL/IU Max ( 2.2 mg/ml, ±S9)　 □ 1)

1) Ministry of Health & Welfare, Japan: Toxicol. Test. Rep. Environ. Chem., Vol..3, pp. 81 (1996) (Tables in English)

● 2-Aminoanthraquinone （2-アミノアントラキノン）
　 117-79-3　　Industry　　223.22

CA CHL/IU Min ( 0.075 mg/ml, -S9), 24h; Poly also; D20= 0.038 (48h) ○ 1)

1) Ministry of Labour, Japan., Mutagen. Test. Data of Exist. Chem. Subst., JETOC (Ed.,), pp 504 (1996) (Tables in English)

● p-Aminoazobenzene （p-アミノアゾベンゼン）
60-09-3　　Industry　　 197.24

DNA E.coli (Repair) Min ( 0.7 mg/well, -S9) ○ 1)

UDS Rat-liver Max (?) □ 2)

CT Rat-embryo Max (?) □ 3)

MNv Mice (ddY) Min ( 150 mg/kg x 2, ip) ○ 4)

1) Ichinotsubo D., et al: Mutation Res.,46, 53-62 (1977)
2) Williams GM: Cancer Res., 37, 1845-1851 (1977)
3) Freeman AE., et al: J. Natl. Cancer Inst., 51, 799 (1973)
4) Morita T., et al.: Mutation Res., 389, 3-122 (1997)

● o-Aminoazobenzene （o-アミノアゾベンゼン）
97-56-3　Industry　　 197.24

MNv Mice (CD-1) Min ( 400 mg/kg , ip) ○ 1)

1) Morita T., et al.: Mutation Res., 389, 3-122 (1997)

● p-Aminobenzaldehyde (p-アミノベンツアルデヒド）(p-formylaniline)
　 556-18-3　　Industry　　121.1

AM Sal./E.coli Max ( 10.0 mg/plate, ±S9) □ 1, 2)

1) Ministry of Health & Welfare, Japan: Toxicol. Test. Rep. Environ. Chem., Vol.. 3 (1996) (Tables in English)
2) Ministry of Labour, Japan: Mutagen. Test. Data of Exist. Chem. Subst., JETOC (Ed.) pp. 203 (1996)(Tables in English

● 2-Aminobenzenethiol (2-Aminothiophenol)
137-07-5　　Industry　　125.19

AM Sal./E.coli Min ( 39.1 μg/plate, -S9); Spe=742 (TA98) ○ 1)

CA CHL/IU Min ( 0.03 mg/ml, +S9), 6-18h; Poly also ○ 2)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1997)(Tables in English)

2) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.), Suppl.4 (2008) (Tables in English)

● 2-Amino-5-chloro-4-methylbenzenesulfonic acid　
　　　88-53-9 　Industry　　258.19

AM Sal./E.coli Max ( 5.0 mg/plate, ±S9) □ 1)

CA CHL/IU Min ( 2.0 mg/ml, -S9), 48h ○ 1)

1) Ministry of Health & Welfare, Japan: Toxicol. Test. Rep. Environ. Chem., Vol. 1 (1994) (Tables in English)

● 2-Amino-4-chlorophenol 　(2-Amino-4-chlorohydroxybenzene)　
　　88-53-9 　Industry　　258.19

AM Sal./E.coli Min ( 1250 μg/plate, +S9); Spe=129 (TA100) ○ 1)

CA CHU/IU Min ( 0.75 μg/ml, -S9), 48h; D20=0.92, Poly also; TR=16000. ◎ 2)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1997) (Tables in English)
2) Ministry of Labour, Japan; Japan Bioassay Res Center, Ann. Rep. (2005)

● 4-Aminodiphenyl （4-アミノジフェニール）　(4-Aminobiphenyl)
　 92-67-1　　Labouratory　169.23　　　　　　　　　　　　　　　　

AM Sal. Min ( 2.5 μg/ml, +S9) ○ 1)

BM E. coli Max ( 1.0 mg/ml, ±S9): □ 2)

UDS Rat Hepatocytes Min ( 16.9μg/ml, +S9)　 ○ 3)

MLA L5178Y Min ( 5.0 μg/ml, +S9) ○ 4)

DNAv Binding Min ( 10.1 mg/kg) ○ 5)

1) Moriya M., et al; J. Natl. Cancer Inst., 63, 977-982 (1979)
2) McMahon RE.,et al.; Cancer Res., 39, 682-693 (1979)
3) Probst GS., et al.; Environ. Mol. Mutagen, 3, 11-32 (1981)
4) Oberly TJ.,et al; Mutation Res., 125, 291-306 (1984)
5) Beland et al.,; Environ. Health Perspect., 49, 125-134 (1983)

US-NTP Genotoxicity Screening：
○Ames Test: ○
○CA Test: ○
○SCE Test: ○
○Drosophila (SLRL) Test: □

IARC Criteria： Group 1
　　　　
【Note】 (Cited from IARC Monographs, Suppl., 6, 1987)

　　No data were available on the genetic and related effects of 4-aminobiphenyl in humans.
　　It formed DNA addducts in the bladder epithelium of dogs and protein adducts in serum albumin of rats treated in vivo. It induced mutation in human fibroblasts and mutation, DNA strand breaks and UDS in cultured rodent cells.
　It was mutagenic to bacteria and induced prophage.　(IARC Monographs, 1, 74, 1971)

● 2-Amino-dipyrido(1,2-a: 3',2'-d) imidazole-HCl (Glu-P-2 HCl)
　 175028-40-7　Laboratory　　220.66

AM Sal. Min (?) ◎ 1)

CA CHL/IU Min( 2.0mg/ml, +S9), 3-21h: D20=4.0, TR=1.5 ○ 2)

1) Nagao M et al., Ann. Rev. Genet., 12, 117-159 (1978)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test in vitro LIC, Tokyo (1998） (Tables in English)

● 2-Aminododecanoic acid （アミノドデカン酸）(Aminolauric acid)
　 693-57-2　　Industry　　215.34

AM Sal./E.coli Max ( 5.0 mg/plate, ±S9) □ 1)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (2000) (Tables in English)

● 2-Aminoethanethiol-HCl
156-57-0　Industry　　　215.34

AM Sal./E.coli Min ( 5.0 mg/plate, -S9); spa=16.2 ○w 1)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) Suppl., 3, pp. 119(2000) (Tables in English)

● N-(Aminoethyl) ethanolamine （N-(アミノエチル)エタノールアミン）
　　　111-41-1　Industry　　104.15

CA CHL/IU Max (1.0 mg/ml,±S9); Poly (Weakly) △ 1)

CAv Mice Max (2.0 g/kg. po); 24h □ 1)

1) Ministry of Health & Welfare, Japan: Toxicol. Test. Rep. Environ. Chem., Vol.4 (1996) (Tables in English)

● Aminoguanidine bicarbonate (Amino-guanidium hydrogen carbonate)

2582-30-1　Industry　　136.11

AM Sal./E.coli Max ( 5.0 mg/plate, ±S9) □ 1)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (2000) (Tables in English)

● 4-Amino-5-hydroxy-2,7-naphthalenedisulfonic acid　(H 酸）　(H acid)
　　5460-09-3　　Industry　　241.30

AM Sal./E.coli Max ( 5.0 mg/plate, ±S9) □ 1)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1996)(Tables in English)

● 7-Amino-4-hydroxy-2-naphthalenesulfonic acid　
(7-アミノ-4-ﾋﾄﾞﾛキシ 2-ナフタレンスルフォン酸）
　　87-02-5　　Industry　　 239.25

AM Sal./ E.coli Min (0.625 mg/plate, +S9) ○ 1)

CA CHL/IU Max (1.5 mg/ml,.-S9), 24; ( ±S9) 6h. □ 1)

CA CHL/IU Min(4.0 mg/ml; +S9, 6h.) ○w 2)

1) Ministry of Health & Welfare, Japan: Toxicol. Test. Rep. Environ. Chem., Vol　.4 (1996) (Tables in English)
2) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1996) (Tables in English)

●Top Page　（トップページ）

●Abbreviation 　（省略記号）　

●Mutagenicity Testing　（変異原性試験）

●Test Systems　(試験法の種類）

●Technical Problems　（技術的問題点）

●List of　Compounds（化合物リスト）

●CAS No. of Compounds (CAS No.）

●Evaluation of Results　（試験結果の評価）

AM	Sal.	Min ( 2.35 mg/plate, ±S9)	○	1-5)
AM	Sal./E. coli	Min ( 2.5 mg/plate, -S9)	○	8)
CA	CHL/IU	Min ( 0.4 mg/ml, -S9):, 24h; D20=0.37, TR=86	○	7)
MB	E. coli	Min (?)	○	1)
YM	Yeast	Min (18.4 x 10^-5M, -S9)	○	6)
MB	S.coelicolor	Min (4.7 mg/plate, -S9)	○	4)
MB	A.nidulans	Max ( 18 mg/plate)	□	4)
UDS	HeLa Cells	Min ( 10^-3 mol/L)	○	5)
DNA	E.coli	Min ( 10 μl)	○	6)
SLRLv	Drosophila	Max ( 150 ppm, ih, for 7h	□	9)
DLv	Rats	Min ( 25 ppm, ih, for 7h/d, for 5d.)	○	9)
CAv	Rats	Max ( 25 ppm, ih, for 7h/d, for 5d.)	□	9)

AM	Sal.	Min ( 10 mg/plate), -S9	○	1)
AM	Sal.	Min ( 10 mg/plate), ±S9	○	2,3)
AM	Sal./E. coli	Min ( 1 mg/ml), ±S9	○	4)
AM	Pneuumococ.	Min ( 0.057 mg/ml), -S9	○	5)
CA	CHO	Min ( 0.15 mg/ml), ±S9	○	6)
CA	Rat, RL4 cells	Min ( 0.037 mg/ml), -S9	○	4)
SCE	CHO	Min ( 0.1 mg/ml), ±S9	○	3)
SCE	V79	Min ( 0.822 mg/ml), -S9, for 2h	○	6)
UDS	Hum. leuko.	Min ( 0.5 mg/ml), -S9, for 4.5h	○	7)
YM	S. cerevisiae	Min ( 0.005 mg/ml), ±S9	○	4)
CT	C3H/10T1/2	Min ( 1000 ppm) -S9, for 21d.	○	8)
CA	CHL/IU	Min ( 0.1 mg/ml, -S9), 24h; D20=0.08; TR=140	○	9)
SLRLv	Drosophila	Min ( 5500 ppm, or), for 3d.	○	3, 10)
CAv	Drosophila	Min ( 5500 ppm, fed), for 3d.	○	11)
MNv	Mice	Max ( 10 ppm, ih, 6h/d, 5d/w), for 102w, ih	□	12)
DLv	Mice	Max ( 2 g/kg, 3d/w, sc), for 8w	□	7)

AM	Sal.	Max (?)	□	1)
CA	CHL/IU	Min ( 0.25 mg/ml, -S9), 24h D20=0.46: TR=24	○	2, 3)

AM	Sal./E.coli	Min ( 312.5 μg/plate、+S９）	○	1)
CA	CHL/IU	Max ( 2.2 mg/ml, ±S9)	□	1)

DNA	E.coli	(Repair) Min ( 0.7 mg/well, -S9)	○	1)
UDS	Rat-liver	Max (?)	□	2)
CT	Rat-embryo	Max (?)	□	3)
MNv	Mice (ddY)	Min ( 150 mg/kg x 2, ip)	○	4)

AM	Sal./E.coli	Min ( 39.1 μg/plate, -S9); Spe=742 (TA98)	○	1)
CA	CHL/IU	Min ( 0.03 mg/ml, +S9), 6-18h; Poly also	○	2)

AM	Sal./E.coli	Max ( 5.0 mg/plate, ±S9)	□	1)
CA	CHL/IU	Min ( 2.0 mg/ml, -S9), 48h	○	1)

AM	Sal./E.coli	Min ( 1250 μg/plate, +S9); Spe=129 (TA100)	○	1)
CA	CHU/IU	Min ( 0.75 μg/ml, -S9), 48h; D20=0.92, Poly also; TR=16000.	◎	2)

AM	Sal.	Min ( 2.5 μg/ml, +S9)	○	1)
BM	E. coli	Max ( 1.0 mg/ml, ±S9):	□	2)
UDS	Rat Hepatocytes	Min ( 16.9μg/ml, +S9)	○	3)
MLA	L5178Y	Min ( 5.0 μg/ml, +S9)	○	4)
DNAv	Binding	Min ( 10.1 mg/kg)	○	5)

CA	CHL/IU	Max (1.0 mg/ml,±S9); Poly (Weakly)	△	1)
CAv	Mice	Max (2.0 g/kg. po); 24h	□	1)

AM	Sal./ E.coli	Min (0.625 mg/plate, +S9)	○	1)
CA	CHL/IU	Max (1.5 mg/ml,.-S9), 24; ( ±S9) 6h.	□	1)
CA	CHL/IU	Min(4.0 mg/ml; +S9, 6h.)	○w	2)